CRISPR Screen Dataset

Rebendenne A (2022) - 13-PMID35879413

H. sapiens

Title: Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs.

Screen Rationale : Increased/decreased resistance to virus
Cell Type : Lung Adenocarcinoma Cell Line [BTO:0001911]
Cell Line : Calu-3 [BTO:0002750, CELLOSAURUS:CVCL_0609]
Phenotype : Response To Virus
Condition : Virus: SARS-CoV-2 ( isolate BetaCoV/France/ IDF0372/2020) (0.005 MOI)
Library : CP1663 custom dCas9-VP64-CRISPRa library (Rebendenne, 2022) | Type: CRISPRa | Format: Pool | Enzyme: dCAS-VP64_Blast (Zhu, 2021) | Methodology: Activation
Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 67 | Full Dataset Size: 495
Experimental Setup : Virus Exposure | Duration: 10 Days | MOI: ~ 0.3
Significance : Authors have indicated that results with Score.1 (Z-score) > 1.96 OR Score.1 (Z-score) < -1.96 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

A secondary CRISPRa activation screen resulting in overexpression of genes was carried out using a custom CRISPRa library (CP1663 ) in Calu-3 cells exposed to the SARS-CoV-2 isolate BetaCoV/France/IDF0372/2020 . CRISPRa activation screens identify genes that when overexpressed result in enriched antiviral gene hits (positive scores) and depleted proviral gene hits (negative scores).

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