CRISPR Screen Dataset
Rebendenne A (2022) - 10-PMID35879413
Title: Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs.
Screen Details:
- Screen Rationale : Increased/decreased resistance to virus
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : A549-ACE2
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 ( isolate BetaCoV/France/ IDF0372/2020) (0.005 MOI)
- Library : CP1663 custom dCas9-VP64-CRISPRa library (Rebendenne, 2022) | Type: CRISPRa | Format: Pool | Enzyme: dCAS-VP64_Blast (Zhu, 2021) | Methodology: Activation
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 36 | Full Dataset Size: 495
- Experimental Setup : Virus Exposure | Duration: 10 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Z-score) > 1.96 OR Score.1 (Z-score) < -1.96 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A secondary CRISPRa activation screen resulting in overexpression of genes was carried out using a custom CRISPRa library (CP1663 ) in A549-ACE2 cells overexpressing ACE2 exposed to the SARS-CoV-2 isolate BetaCoV/France/IDF0372/2020 . CRISPRa activation screens identify genes that when overexpressed result in enriched antiviral gene hits (positive scores) and depleted proviral gene hits (negative scores).
Score Distribution
< 5.66494582
> -5.883946039