CRISPR Screen Dataset
Lu J (2022) - 1-PMID35570209
Title: Inwardly rectifying potassium channels mediate polymyxin-induced nephrotoxicity.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Kidney Cell Line [BTO:0000067]
- Cell Line : HK-2 [BTO:0003575, CELLOSAURUS:CVCL_0302]
- Phenotype : Response To Chemicals
- Condition : polymyxin B (25.0 µM) [ChEBI]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 86 | Full Dataset Size: 20,109
- Experimental Setup : Drug Exposure | Duration: 28 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (FDR) > 1.3 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes provide increased resistance to polymyxin B upon knock-out
Score Distribution
< 6.8232
> -6.1709