CRISPR Screen Dataset
Feng X (2022) - 17-PMID35559673
Title: Genome-wide CRISPR screens using isogenic cells reveal vulnerabilities conferred by loss of tumor suppressors.
Screen Details:
- Screen Rationale : Cell-essential genes in a TP53BP1 KO background
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293-A [BTO:0002524, CELLOSAURUS:CVCL_6910]
- Phenotype : Cell Proliferation
- Condition : Mutation: TP53BP1 (ETG7158) KO
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : BAGEL | Number of Hits: 1,988 | Full Dataset Size: 18,053
- Experimental Setup : Timecourse | Duration: 21 Days | MOI: ~ 0.25
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for genes essential in a TP53BP1 knockout HEK293A cell line
Score Distribution
< 77.998
> -52.008