CRISPR Screen Dataset
Juhász S (2020) - 1-PMID33355125
Title: The chromatin remodeler ALC1 underlies resistance to PARP inhibitor treatment.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Response To Chemicals
- Condition : Olaparib (3 µM) [PubChem]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 792 | Full Dataset Size: 17,809
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Z-score) < -2.0 OR Score.1 (Z-score) > 2.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant negative Z-score show increased sensitivity to PARPi olaparib upon KO, genes with a significant positive Z-score show decreased sensitivity to PARPi olaparib upon KO
Score Distribution
< 7.89
> -5.55