CRISPR Screen Dataset
Grodzki M (2022) - 9-PMID35086559
Title: Genome-scale CRISPR screens identify host factors that promote human coronavirus infection.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T/ACE2 [CELLOSAURUS:CVCL_C9BE]
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 (Strain UF-1) (0.03 MOI) [GENBANK:MT295464.1]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 61 | Full Dataset Size: 19,115
- Experimental Setup : Virus Exposure | Duration: 15 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (FDR) < 0.25 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR screen was carried out in HEK293T-hACE2 cells to identify host factors required for SARS-CoV-2 infection.
- (File 7 HEKSARS Reinfect MOI 0.03: MAGeCK HEK293T-Ace2 SARS-CoV-2 Low MOI 0.03 re- infection day 5 (15 total days))
Score Distribution
< 1
> 0.000381