CRISPR Screen Dataset
Kratzel A (2021) - 4-PMID34962926
Title: A genome-wide CRISPR screen identifies interactors of the autophagy pathway as conserved coronavirus targets.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Hepatoma Cell Line [BTO:0000578]
- Cell Line : HuH-7 [BTO:0001950, CELLOSAURUS:CVCL_0336, DEPMAP:ACH-000480]
- Phenotype : Response To Virus
- Condition : Virus: HCoV-229E (Human coronavirus 229E) (0.1 MOI) [NCBI Taxid 11137]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 377 | Full Dataset Size: 21,668
- Experimental Setup : Virus Exposure | Duration: 14 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (p-Value) <= 0.05 AND Score.2 (Log2FC) >= 2.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-scale loss-of-function CRISPR screen was performed to identify host dependency factors (HDFs) essential for HCoV-229E coronavirus infection (second-best LFC method).
Score Distribution
< 1
> 0.000000228