CRISPR Screen Dataset
Sherman EJ (2022) - 2-PMID35231079
Title: Identification of cell type specific ACE2 modifiers by CRISPR screening.
Screen Details:
- Screen Rationale : Increased/decreased ACE2 cell surface abundance
- Cell Type : Hepatoma Cell Line [BTO:0000578]
- Cell Line : HuH-7 [BTO:0001950, CELLOSAURUS:CVCL_0336, DEPMAP:ACH-000480]
- Phenotype : Protein/peptide Distribution [APO:0000209]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 48 | Full Dataset Size: 21,661
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Rank) < 25.0 OR Score.3 (Rank) < 25.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR screen was carried out in liver-derived HuH7 ACE2-enriched cells to identify cell type specific positive and negative regulators of ACE2 surface abundance. The authors listed the top ranked gene hits in Table S1.
Score Distribution
< 21661
> 1