CRISPR Screen Dataset
Vichas A (2021) - 2-PMID34373451
Title: Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.
Screen Details:
- Screen Rationale : Increased/decreased sensitivity to drug in an EGFR-T790M/L858R mutant background
- Cell Type : Lung Adenocarcinoma Cell Line [BTO:0001911]
- Cell Line : PC-9 [BTO:0005518, CELLOSAURUS:CVCL_B260]
- Phenotype : Response To Chemicals
- Condition : Mutation: EGFR (ETG1956) T790M/L858R| erlotinib (40.0 nM)
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,487 | Full Dataset Size: 19,363
- Experimental Setup : Drug Exposure | Duration: 12 Doublings | MOI: < 0.3
- Significance : Authors have indicated that results with Score.1 (CRISPR Score (CS)) < -0.5 AND Score.2 (Log10 (p-value)) > 1.3 OR Score.1 (CRISPR Score (CS)) > 0.5 AND Score.2 (Log10 (p-value)) > 1.3 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant negative CRISPR score confer decreased growth in the presence of erlotinib in an EGFR mutant background upon KO , genes with a positive CRISPR score confer increased growth in the presence of erlotinib in an EGFR mutant background upon KO
Score Distribution
< 0.918263579
> -3.940660246