CRISPR Screen Dataset
Xu P (2021) - 6-PMID34155407
Title: Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs.
Screen Details:
- Screen Rationale : Cell-essential genes and growth restrictive genes
- Cell Type : Retinal Pigment Epithelium Cell Line [BTO:0002334]
- Cell Line : hTERT-RPE1 [BTO:0004790, CELLOSAURUS:CVCL_4388]
- Phenotype : Cell Proliferation
- Condition : Mutation: TP53 (ETG:7157) KO
- Library : BARBEKO sgRNA library [AddGene] | Type: Cytosine Base Editing-Mediated Gene KnockOut | Format: Pool | Enzyme: AncBE4max | Methodology: Knockout
- Analysis Method : ZFCiBAR | Number of Hits: 1,878 | Full Dataset Size: 17,833
- Experimental Setup : Timecourse | Duration: 15 Days | MOI: = 3.0
- Significance : Authors have indicated that results with Score.1 (FS (Fitness Score)) < -3.0 OR Score.1 (FS (Fitness Score)) > 3.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors used their newly developed DSB-independent, genome-wide CRISPR screening method, termed iBARed cytosine base editing-mediated gene KO (BARBEKO).
- Authors used a cytosine base editing enzyme to generate KOs instead of a method relying on DSBs.
Score Distribution
< 14.71949968
> -17.54625051