CRISPR Screen Dataset
Xu P (2021) - 3-PMID34155407
Title: Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs.
Screen Details:
- Screen Rationale : Cell-essential genes and growth restrictive genes
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Cell Proliferation
- Library : BARBEKO sgRNA library [AddGene] | Type: Cytosine Base Editing-Mediated Gene KnockOut | Format: Pool | Enzyme: AncBE4max | Methodology: Knockout
- Analysis Method : ZFCiBAR | Number of Hits: 1,149 | Full Dataset Size: 17,833
- Experimental Setup : Timecourse | Duration: 30 Days | MOI: = 3.0
- Significance : Authors have indicated that results with Score.1 (FS (Fitness Score)) < -3.0 OR Score.1 (FS (Fitness Score)) > 3.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors used their newly developed DSB-independent, genome-wide CRISPR screening method, termed iBARed cytosine base editing-mediated gene KO (BARBEKO).
- Authors used a cytosine base editing enzyme to generate KOs instead of a method relying on DSBs, allowing screening at higher MOI with smaller cell populations
Score Distribution
< 5.541484184
> -7.645096571