CRISPR Screen Dataset
Damnernsawad A (2022) - 1-PMID33375770
Title: Genome-wide CRISPR screen identifies regulators of MAPK and MTOR pathways mediating sorafenib resistance in acute myeloid leukemia.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Acute Myeloid Leukemia Cell Line [BTO:0001883]
- Cell Line : MOLM-13 [BTO:0004175, CELLOSAURUS:CVCL_2119]
- Phenotype : Response To Chemicals
- Condition : sorafenib (50.0 nM) [ChEBI]
- Library : Human Improved Genome-wide Knockout CRISPR Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 985 | Full Dataset Size: 18,010
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) <= 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in increased resistance to sorafenib
Score Distribution
< 0.047
> 0.00000092