CRISPR Screen Dataset
Mei H (2021) - 2-PMID34686207
Title: Surfaceome CRISPR screen identifies OLFML3 as a rhinovirus-inducible IFN antagonist.
Screen Details:
- Screen Rationale : Cell-essential genes and growth restrictive genes
- Cell Type : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Cell Line : H1HeLa [CLO:0003586, CELLOSAURUS:CVCL_3334]
- Phenotype : Cell Proliferation
- Library : Surfaceome Library | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 17 | Full Dataset Size: 2,344
- Experimental Setup : Timecourse | Duration: 10 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.001 OR Score.4 (FDR) < 0.001 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant negative FDR represent genes whose KO results in decreased cellular proliferation, genes with a significant positive FDR represent genes whose KO results in increased cellular proliferation
- A surfaceome focused library
Score Distribution
< 0.99997
> 1.2e-18