CRISPR Screen Dataset
Zhu S (2021) - 1-PMID34431042
Title: Genome-wide CRISPR activation screen identifies candidate receptors for SARS-CoV-2 entry.
Screen Details:
- Screen Rationale : Decreased resistance to virus
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T-CRISPRa [CELLOSAURUS:CVCL_C9DX]
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 pseudovirus + EGFP marker (Zhu, 2021)
- Library : CRISPRa sgRNA-eBAR library (Zhu, 2021) | Type: CRISPRa | Format: Pool | Enzyme: dCAS-VP64_Blast (Zhu, 2021) | Methodology: Activation
- Analysis Method : eBAR-analyzer (Zhu, 2021) | Number of Hits: 190 | Full Dataset Size: 19,481
- Experimental Setup : Virus Exposure | MOI: ~ 10
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- To identify novel host factors that enable SARS-CoV-2 infection, a genome-wide CRISPRa gain-of-function screen using a SARS-CoV-2 spike-pseudotyped virus with an EGFP marker was carried out in HEK293T cells. Infected cells were sorted by FACS based on EGFP intensity which represented the strength of candidate host factors facilitating virus entry. The authors used a significance cutoff of RRA score < 0.001 . Gene hits are provided for cells with the top 2% of EGFP fluorescence intensity.
Score Distribution
< 1
> 0.00000000185