CRISPR Screen Dataset
Akinci E (2022) - 3-CUSTOM00000017
Title: Elucidation of remdesivir cytotoxicity pathways through genome-wide CRISPR-Cas9 screening and transcriptomics
Screen Details:
- Screen Rationale : Increased/decreased resistance to drug
- Cell Type : Colonic Adenocarcinoma Cell Line [BTO:0001913]
- Cell Line : HT-29 [BTO:0000182, CELLOSAURUS:CVCL_0320]
- Phenotype : Response To Chemicals
- Condition : Remdesivir (1.67 µM) [ChEBI]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MAGeCK-MLE | Number of Hits: 1,230 | Full Dataset Size: 19,114
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: ~ 0.5
- Significance : Authors have indicated that results with Score.1 (Beta Score) < 0.0 AND Score.2 (FDR) < 0.01 OR Score.1 (Beta Score) > 0.0 AND Score.2 (FDR) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A CRISPR screen was carried out to identify affected genes in the remdesivir treated cells. The top gene hits with an FDR < 0.01 are noted. Negative beta scores indicate depleted genes and positive beta scores indicate enriched genes.
Score Distribution
< 1.3344
> -1.7092