CRISPR Screen Dataset

Tian (2021) - 2-CUSTOM00000007

H. sapiens

Title: BRD2 inhibition blocks SARS-CoV-2 infection in vitro by reducing transcription of the host cell receptor ACE2

Screen Rationale : Increased/decreased protein binding
Cell Type : Lung Adenocarcinoma Cell Line [BTO:0001911]
Cell Line : Calu-3 [BTO:0002750, CELLOSAURUS:CVCL_0609]
Phenotype : Protein Binding
Condition : Transferrin receptor (TFRC/CD71) (10.0 nM)
Library : H1 CRISPRi (Horlbeck, 2016) | Type: CRISPRi | Format: Pool | Enzyme: dCas9-BFP-KRAB | Methodology: Inhibition
Analysis Method : MAGeCK-iNC | Number of Hits: 369 | Full Dataset Size: 2,555
Experimental Setup : Transferrin receptor (TFRC/CD71) exposure
Significance : Authors have indicated that results with Score.1 (MaGeCK Score) < 0.0 AND Score.2 (p-Value) < 0.05 OR Score.1 (MaGeCK Score) > 0.0 AND Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

To show the specificity of the Spike-RBD CRISPRi screen, a parallel screen was run with an antibody agains the transferrin receptor (TFRC/CD71) to control for factors that generally affect protein trafficking or protein binding to the cell surface. Genes were knocked down by CRISPRi and those that decreased (negative score) or increased (positive score) TFRC levels were identified, including RAB7A which is essential for trafficking of TFRC to the cell surface.

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