CRISPR Screen Dataset

Biering SB (2022) - 2-PMID35879412

H. sapiens

Title: Genome-wide bidirectional CRISPR screens identify mucins as host factors modulating SARS-CoV-2 infection.

Screen Rationale : Decreased resistance to virus
Cell Type : Lung Adenocarcinoma Cell Line [BTO:0001911]
Cell Line : Calu-3 [BTO:0002750, CELLOSAURUS:CVCL_0609]
Phenotype : Response To Virus
Condition : Virus: SARS-CoV-2 (isolate USA-WA1/2020) (0.05 MOI)
Library : Human CRISPR Activation Pooled Library (Calabrese) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: dCas9-VP64 + PP7-P65-HSF1 | Methodology: Activation
Analysis Method : MaGeCK | Number of Hits: 43 | Full Dataset Size: 18,884
Experimental Setup : Virus Exposure | Duration: 5 Days | MOI: = 0.3
Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

A genome-scale gain-of-function CRISPR screen was performed to identify proviral host factors modulating SARS-CoV-2 infection (GOF-depleted data set).

< 1

> 0.000000000000171