CRISPR Screen Dataset
Mimura K (1970) - 1-PMID34000301
Title: Genome-wide CRISPR screening reveals nucleotide synthesis negatively regulates autophagy.
Screen Details:
- Screen Rationale : Negative regulators of autophagy
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 17 | Full Dataset Size: 21,686
- Experimental Setup : Timecourse | Duration: 2 Weeks | MOI: ~ 0.4
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.001 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors used the GFP-LC3-RFP autophagic flux reporter to screen for negative regulators of autophagy
Score Distribution
< 1
> 0.000000000000247