CRISPR Screen Dataset
Wheeler LJ (2019) - 1-PMID31437751
Title: Multi-Omic Approaches Identify Metabolic and Autophagy Regulators Important in Ovarian Cancer Dissemination.
Screen Details:
- Screen Rationale : Regulation of anoikis escape
- Cell Type : Ovarian Cancer Cell Line [BTO:0000811, EFO:0002394]
- Cell Line : PEO1 [EFO:0005445, CELLOSAURUS:CVCL_2686]
- Phenotype : Cell Proliferation
- Condition : Culture in suspension vs. adherent conditions
- Library : GECKO | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DESeq2 | Number of Hits: 2,206 | Full Dataset Size: 18,080
- Experimental Setup : Other | Duration: 10 Days | MOI: ~ 0.5
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- Genes with a negative log2fc show decreased growth in suspension upon KO, suggesting that the targeted genes are required for survival in the context of anchorage independence.
Score Distribution
< 0.008837541
> 3.49e-28