CRISPR Screen Dataset
Wang X (2021) - 2-PMID34582788
Title: In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target.
Screen Details:
- Screen Rationale : Regulation of cancer cell response to immune system
- Cell Type : Breast Adenocarcinoma Cell Line [BTO:0001912]
- Cell Line : 4T1 [BTO:0004311, CELLOSAURUS:CVCL_0125]
- Phenotype : Tumorigenicity
- Condition : BALB/c VS. BALB/c Foxn1nu/nu hosts
- Library : MusCK | Type: CRISPRn | Format: in vivo | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 12 | Full Dataset Size: 4,776
- Experimental Setup : Implantation to Mouse Model | Duration: 16 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.001 OR Score.4 (p-Value) < 0.001 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors inspected sgRNAs depleted from tumors in wild-type immunocompetent hosts compared to nude immunodeficient mice to reveal key genes promoting immune evasion in 4T1 cancer cells
- Significant negative score indicates depletion of sgRNA in wt compared to immuno-deficent host, significant positive score indicates enrichment of sgRNA in wt compared to immuno-deficient host
Score Distribution
< 0.99978
> 0.00000629