CRISPR Screen Dataset
Ramaker RC (2021) - 2-PMID34049503
Title: Pooled CRISPR screening in pancreatic cancer cells implicates co-repressor complexes as a cause of multiple drug resistance via regulation of epithelial-to-mesenchymal transition.
Screen Details:
- Screen Rationale : Increased resistance to drug
- Cell Type : Pancreatic Cancer Cell Line [BTO:0000794]
- Cell Line : PANC-1 [BTO:0000304, CELLOSAURUS:CVCL_0480]
- Phenotype : Response To Chemicals
- Condition : Irinotecan (500.0 nM) [ChEBI]
- Library : SAM v1 (Zeomycin) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: SAM (NLS-dCas9-VP64/MS2-p65-HSF1) | Methodology: Activation
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 3,869 | Full Dataset Size: 22,098
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Activation of the hit gene confers resistance to irinotecan in a PANC-1 cell background
Score Distribution
< 9.591490143
> -21.75764836