CRISPR Screen Dataset
Sakuma C (2021) - 1-PMID34066520
Title: Identification of SYS1 as a Host Factor Required for Shiga Toxin-Mediated Cytotoxicity in Vero Cells.
Screen Details:
- Screen Rationale : Increased resistance to toxin
- Cell Type : African Green Monkey Kidney Cell Line
- Cell Line : Vero-E6|VERO C1008 [BTO:0004755, COVOC:0020002, CELLOSAURUS:CVCL_0574]
- Phenotype : Response To Toxin
- Condition : Shiga Toxin Stx1 (20 pg/mL)
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 234 | Full Dataset Size: 22,298
- Experimental Setup : Toxin Exposure | Duration: 24 Days | MOI: ~ 0.2
- Significance : Authors have indicated that results with Score.3 (p-Value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened African green monkey cells (Vero C1008) using the human Gecko v2 library.
Score Distribution
< 0.99986
> 3.1796e-17