CRISPR Screen Dataset
Zheng A (2019) - 1-PMID31903165
Title: CRISPR/Cas9 genome-wide screening identifies KEAP1 as a sorafenib, lenvatinib, and regorafenib sensitivity gene in hepatocellular carcinoma.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Huh-7 Cell [BTO:0001950]
- Cell Line : HUH-7 SR [CELLOSAURUS:CVCL_C8ZK]
- Phenotype : Response To Chemicals
- Condition : sorafenib (5 µM) [ChEBI]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1 | Full Dataset Size: 20,471
- Experimental Setup : Drug Exposure | Duration: 12 Doublings | MOI: = 0.3
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in enrichment in the presence of sorafenib
Score Distribution
< 1
> 0.0000000000936