CRISPR Screen Dataset

Schukur L (2020) - 1-PMID33208877

H. sapiens

Title: Identification of the HECT E3 ligase UBR5 as a regulator of MYC degradation using a CRISPR/Cas9 screen.

Screen Rationale : Regulators of MYC stability
Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
Cell Line : HEK293-A [BTO:0002524, CELLOSAURUS:CVCL_6910]
Phenotype : Protein/peptide Accumulation [APO:0000149]
Library : CRISPRn UPS targeted library (Thoma, 2020) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : EdgeR | Number of Hits: 46 | Full Dataset Size: 1,049
Experimental Setup : Timecourse | Duration: 7 Days | MOI: = 0.3
Significance : Authors have indicated that results with Score.1 (Log2FC) > 0.5 AND Score.2 (RSA) < -0.5 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Cells were infected with library, grown for 7 days and sorted for high GFP expression, suggesting increased MYC levels. KO of the hit gene results in stabilization of MYC

< 1.721

> -0.803