CRISPR Screen Dataset
Ohnstad AE (2020) - 3-PMID33226137
Title: Receptor-mediated clustering of FIP200 bypasses the role of LC3 lipidation in autophagy.
Screen Details:
- Screen Rationale : Regulators of autophagy in an ATG10 KO background
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Mutation: ATG10 (ETG83734) KO
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 197 | Full Dataset Size: 19,113
- Experimental Setup : Timecourse | Duration: 12 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.1 (Beta Score) > 0.5 OR Score.1 (Beta Score) < -0.5 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors performed screen in an ATG10 KO background in which LC3 lipidation is inhibited.
- Using a tf-NBR1 reporter in which fluorescent ratios can be used to indicate functional autophagy the authors screened for genes whose KO resulted in enhanced defective autophagy of tf-NBR1 (positive beta score) in an ATG10KO background and screened for genes whose KO resulted in suppressed defective autophagy of tf-NBR1 (negative beta score) in an ATG10KO background
Score Distribution
< 2.0897
> -1.15127