CRISPR Screen Dataset
Gul N (2019) - 1-PMID31604991
Title: The MTH1 inhibitor TH588 is a microtubule-modulating agent that eliminates cancer cells by activating the mitotic surveillance pathway.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Large Cell Lung Cancer Cell Line [BTO:0002205]
- Cell Line : NCI-H460 [BTO:0002207, CELLOSAURUS:CVCL_0459]
- Phenotype : Response To Chemicals
- Condition : TH588 (4 µM) [ChemSpider]
- Library : Kinase and Cell Cycle Subpools from Human CRISPR Knockout Pooled Libraries [AddGene, AddGene, AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MAGeCK-MLE | Number of Hits: 42 | Full Dataset Size: 1,490
- Experimental Setup : Drug Exposure | Duration: 18 Days
- Significance : Authors have indicated that results with Score.1 (Log2FC) > 0.0 AND Score.3 (FDR) < 0.2 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for mutations that rescue the cytotoxic effect of TH588
Score Distribution
< 3.5414
> -2.2247