CRISPR Screen Dataset
Baggen J (2021) - 4-PMID33686287
Title: Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Hepatoma Cell Line [BTO:0000578]
- Cell Line : HuH-7 [BTO:0001950, CELLOSAURUS:CVCL_0336, DEPMAP:ACH-000480]
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) (0.1 MOI) [NCBI Taxid 2697049]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 378 | Full Dataset Size: 18,227
- Experimental Setup : Virus Exposure | Duration: 14 Days | MOI: ~ 0.2
- Significance : Authors have indicated that results with Score.1 (Log2FC) > 1.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The low stringency screen involved infecting cells to identify pro-viral host genes that confer resistance under mild selection when knocked out. These are indicated by a positive score for enrichment with a Log2FC >= 1.0. Genes having a Log2FC >= 0.5 in the uninfected control screen were removed from the list. (Supplementary Table 11)
Score Distribution
< 2.275804933
> -1.745740297