CRISPR Screen Dataset
Ji L (2019) - 1-PMID31519875
Title: USP7 inhibits Wnt/β-catenin signaling through promoting stabilization of Axin.
Screen Details:
- Screen Rationale : Negative regulators of Wnt signaling
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Wnt3a conditioned medium (5.0 %)
- Library : CRISPRn (Cong, 2019) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 3,855 | Full Dataset Size: 17,986
- Experimental Setup : Timecourse | Duration: 15 Days | MOI: = 0.5
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors used an STF-GFP reporter to screen for cells with high Wnt signalling, then looked at genes depleted in this population suggesting KO of the gene results in high signaling and the gene normally functions as a negative regulator or WNT signaling.
Score Distribution
< -0.00072525
> -14.37154496