CRISPR Screen Dataset
Liang Y (2021) - 1-PMID33523994
Title: TRIM26 is a critical host factor for HCV replication and contributes to host tropism.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Huh-7 Cell [BTO:0001950]
- Cell Line : Huh-7.5 [BTO:0004126, CELLOSAURUS:CVCL_7927]
- Phenotype : Response To Virus
- Condition : Virus: Hepatitis C Virus (0.1 MOI) [NCBI Taxid 356113]
- Library : CRISPRn (Zhong, 2021) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RIGER | Number of Hits: 14 | Full Dataset Size: 17,766
- Experimental Setup : Virus Exposure | Duration: 30 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (RIGER score) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for genes whose KO results in decreased infection by HCV, suggesting the gene is essential for HCV infection
Score Distribution
< 2.33
> 0.00005065