CRISPR Screen Dataset
Du R (2021) - 1-PMID33676427
Title: Inhibition of ERAD synergizes with FTS to eradicate pancreatic cancer cells.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Pancreatic Ductal Adenocarcinoma Cell Line [BTO:0003883]
- Cell Line : 4292 KRASG12D/p53R172H
- Phenotype : Response To Chemicals
- Condition : salirasib (Farnesyl Thiosalicylic Acid) (100.0 µM) [ChemSpider]
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,943 | Full Dataset Size: 19,674
- Experimental Setup : Drug Exposure | Duration: 12 Days
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes with a negative beta score causes decreased proliferation in the presence of FTS, KO of hit genes with a positive beta score causes increased proliferation in the presence of FTS
Score Distribution
< 3.0964
> -2.3321