CRISPR Screen Dataset
Liang JR (2020) - 1-PMID32160526
Title: A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.
Screen Details:
- Screen Rationale : Regulators of ER-autophagy
- Cell Type : Colorectal Cancer Cell Line [BTO:0001616]
- Cell Line : HCT 116 [BTO:0001109, CELLOSAURUS:CVCL_0291]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Prolonged amino acid starvation (16 h) using Earl’s buffered saline solution (EBSS)
- Library : CRISPRi-v2 (inhibition) [AddGene] | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 200 | Full Dataset Size: 18,905
- Experimental Setup : Other | Duration: 11 Days
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- The authors performed a genome-wide CRISPR interference (CRISPRi) reporter-based screen to identify genes involved in autophagy mediated by the endoplasmic reticulum (ER-phagy).
- Genes with a negative enhanced/inhibition fold change repressed ER-phagy upon inhibition, genes with a positive enhanced/inhibiton fold change enhanced ER-phagy upon inhibition
Score Distribution
< 3.619
> -5.368