CRISPR Screen Dataset
Liu X (2020) - 2-PMID32355287
Title: ERCC6L2 promotes DNA orientation-specific recombination in mammalian cells.
Screen Details:
- Screen Rationale : Decreased resistance to drug
- Cell Type : Lymphoma Cell Line [BTO:0000104]
- Cell Line : CH12F3 [BTO:0006297, CELLOSAURUS:CVCL_E067]
- Phenotype : Response To Chemicals
- Condition : A-966492 (2.5 µM)
- Library : CRISPRn targeted mouse library (Liu, 2020) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 51 | Full Dataset Size: 566
- Experimental Setup : Drug Exposure | Duration: 12 Days
- Significance : Authors have indicated that results with Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line.
Score Distribution
< 0.99981
> 0.0000049629