CRISPR Screen Dataset
Kinnebrew M (2019) - 1-PMID31657721
Title: Cholesterol accessibility at the ciliary membrane controls hedgehog signaling.
Screen Details:
- Screen Rationale : Negative regulators of the HH pathway
- Cell Type : Embryonic Fibroblast Cell Line [BTO:0001958]
- Cell Line : NIH 3T3 [BTO:0000944, CELLOSAURUS:CVCL_0594]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Sonic hedgehog protein (mouse) (3.2 nM) [Uniprot]
- Library : Targeted lipid-related genes library (Rohatgi, 2019) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 253 | Full Dataset Size: 1,239
- Experimental Setup : Ligand Exposure | Duration: 24 Hours
- Significance : Authors have indicated that results with Score.1 (p-Value) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- From the paper: we treated cells with a low, sub-saturating concentration of SHH (LoSHH) that activated the reporter to <10% of maximal strength and selected super-responders, cells with the top 5% of GLI-GFP fluorescence
Score Distribution
< 1
> 0.00000344