CRISPR Screen Dataset
Hundley FV (2021) - 11-PMID33539788
Title: A comprehensive phenotypic CRISPR-Cas9 screen of the ubiquitin pathway uncovers roles of ubiquitin ligases in mitosis.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Chronic Myelogenous Leukemia Cell Line []
- Cell Line : HAP-1 [EFO:0007598, CELLOSAURUS:CVCL_Y019]
- Phenotype : Response To Chemicals
- Condition : Nicotinamide (12 mM) [ChemSpider]
- Library : CRISPRn (Hundley, 2021) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 6 | Full Dataset Size: 697
- Experimental Setup : Drug Exposure | Duration: 8 Days | MOI: ~ 0.4
- Significance : Authors have indicated that results with Score.1 (Log2FC) <= -0.3 AND Score.2 (p-Value) <= 0.01 OR Score.1 (Log2FC) >= 0.3 AND Score.2 (p-Value) <= 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors screened for human ubiquitin pathway genes, including 629 E3 ubiquitin ligases and 56 deubiquitinating enzymes (DUBs), whose knockout led to increased resistance to the specified drug (positive log2fc) or increased sensitivity to the drug (negative log2fc).
- Biological process: Transcription/RNA processing
Score Distribution
< 0.4671951851
> -0.4605451558