CRISPR Screen Dataset
Zhang H (2020) - 2-PMID33172989
Title: Type I interferon remodels lysosome function and modifies intestinal epithelial defense.
Screen Details:
- Screen Rationale : Increased resistance to bacteria
- Cell Type : Colonic Adenocarcinoma Cell Line [BTO:0001913]
- Cell Line : HT-29 [BTO:0000182, CELLOSAURUS:CVCL_0320]
- Phenotype : Response To Bacteria
- Condition : Salmonella enterica serovar Typhimurium (300.0 MOI) [NCBI Taxid 90371]
- Library : Avana | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 147 | Full Dataset Size: 19,647
- Experimental Setup : Bacteria Exposure | Duration: 4 Passages
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.001 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in improved growth in the presence of Salmonella enterica serovar Typhimurium, suggesting normal function of the gene is needed for cytotoxicity
- The authors performed the screen using two Avana sub-libraries, these are the results from sub-library B
Score Distribution
< 1
> 1.02e-16