CRISPR Screen Dataset
Henkel L (2020) - 2-PMID33228647
Title: Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Chronic Myelogenous Leukemia Cell Line []
- Cell Line : HAP-1 [EFO:0007598, CELLOSAURUS:CVCL_Y019]
- Phenotype : Cell Proliferation
- Library : Heidelberg (HD) CRISPR library | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : BAGEL | Number of Hits: 1,925 | Full Dataset Size: 18,343
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: ~ 0.2
- Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 6.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors performed their screen using clones of a single HAP1 cell (SCC12) with high Cas9 editing activity for increased sensitivity.
Score Distribution
< 57.718
> -106.496