CRISPR Screen Dataset
Gao G (2019) - 1-PMID30916320
Title: PRMT1 loss sensitizes cells to PRMT5 inhibition.
Screen Details:
- Screen Rationale : Increased sensitivity to drug
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : NCI-H2171 [BTO:0005978, CELLOSAURUS:CVCL_1536]
- Phenotype : Response To Chemicals
- Condition : EPZ015666 (GSK3235025) (0.5 µM) [PubChem]
- Library : EpiC library | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MoPAC | Number of Hits: 8 | Full Dataset Size: 1,118
- Experimental Setup : Drug Exposure | Duration: 18 Days | MOI: < 0.3
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.2 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in increased sensitivity to the potent and specific PRMT5 small molecule inhibitor (EPZ015666), suggesting the gene functions synergistically with PRMT5
Score Distribution
< 3.519893069
> -4.458899426