CRISPR Screen Dataset

Shu S (2020) - 8-PMID32416067

H. sapiens

Title: Synthetic Lethal and Resistance Interactions with BET Bromodomain Inhibitors in Triple-Negative Breast Cancer.

Screen Rationale : Increased/decreased drug resistance
Cell Type : Breast Cancer Cell Line [BTO:0000356, EFO:0002885]
Cell Line : SUM159R [CELLOSAURUS:CVCL_C9AN]
Phenotype : Response To Chemicals
Condition : JQ1 (20 µM) [ChEBI]
Library : Liu Human CRISPR Knockout Library (CRISPRn H1 H2, H1/H2) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 123 | Full Dataset Size: 18,460
Experimental Setup : Drug Exposure | Duration: 5 Passages | MOI: = 0.3
Significance : Authors have indicated that results with Score.1 (Log10 (p-value)) <= -3.0 OR Score.1 (Log10 (p-value)) >= 3.0 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Genes with a negative score are depleted upon KO and represent genes whose loss increases sensitivity to JQ1 in a BBDI-resistant cell line.
Genes with a positive score are enriched upon KO and represent genes whose loss decreases sensitivity to JQ1 in a BBDI-resistant cell line.

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