CRISPR Screen Dataset
Shu S (2020) - 6-PMID32416067
Title: Synthetic Lethal and Resistance Interactions with BET Bromodomain Inhibitors in Triple-Negative Breast Cancer.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Breast Cancer Cell Line [BTO:0000356, EFO:0002885]
- Cell Line : SUM159PT [BTO:0004928, CELLOSAURUS:CVCL_5423]
- Phenotype : Response To Chemicals
- Condition : JQ1 (1 µM) [ChEBI]
- Library : Liu Human CRISPR Knockout Library (CRISPRn H1 H2, H1/H2) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 248 | Full Dataset Size: 18,460
- Experimental Setup : Drug Exposure | Duration: 5 Passages | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Log10 (p-value)) <= -3.0 OR Score.1 (Log10 (p-value)) >= 3.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a negative score are depleted upon KO and represent genes whose loss increases sensitivity to JQ1.
- Genes with a positive score are enriched upon KO and represent genes whose loss decreases sensitivity to JQ1.
Score Distribution
< 6.589796583
> -6.589796583