CRISPR Screen Dataset
Holmes DL (2020) - 2-PMID33122441
Title: A CRISPR-Cas9 screen identifies mitochondrial translation as an essential process in latent KSHV infection of human endothelial cells.
Screen Details:
- Screen Rationale : Decreased resistance to virus
- Cell Type : Microvascular Endothelial Cell Line [BTO:0003124]
- Cell Line : TIME [CELLOSAURUS:CVCL_0047]
- Phenotype : Response To Virus
- Condition : Virus: Human gammaherpesvirus 8 (Kaposi's sarcoma-associated herpesvirus KSHV) [NCBI Taxid 37296]
- Library : Human Activity-optimized genome-wide library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,631 | Full Dataset Size: 11,468
- Experimental Setup : Virus Exposure | Duration: 8 Days | MOI: = 0.6
- Significance : Authors have indicated that results with Score.4 (Log2FC) > 2.75 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in decreased viability in cells latently infected with KSHV
Score Distribution
< 0.99999
> 0.00000384