CRISPR Screen Dataset
To TL (2019) - 2-PMID31730859
Title: A Compendium of Genetic Modifiers of Mitochondrial Dysfunction Reveals Intra-organelle Buffering.
Screen Details:
- Screen Rationale : Increased/decreased resistance to drug
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Cell Proliferation
- Condition : Antimycin and Oligomycin (10 nM) [ChEBI, ChEBI]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Delta Z scores | Number of Hits: 80 | Full Dataset Size: 20,115
- Experimental Setup : Drug Exposure | Duration: 15 Days | MOI: ~ 0.5
- Significance : Authors have indicated that results with Score.1 (Z-score) < -2.4 OR Score.1 (Z-score) > 4.8 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- CRISPR screens were carried out using different small molecule mitochondrial inhibitors to study genetic pathways that aggravate or suppress mitochondrial dysfunction.
Score Distribution
< 8.993463327
> -4.620435899