CRISPR Screen Dataset
Orvedahl A (2019) - 1-PMID31346084
Title: Autophagy genes in myeloid cells counteract IFNγ-induced TNF-mediated cell death and fatal TNF-induced shock.
Screen Details:
- Screen Rationale : Increased ligand resistance
- Cell Type : Microglial Cell Line [BTO:0003349]
- Cell Line : BV-2 [BTO:0003350, CELLOSAURUS:CVCL_0182]
- Phenotype : Cell Proliferation
- Condition : Interferon gamma (10.0 units/mL)
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : STARS | Number of Hits: 14 | Full Dataset Size: 20,147
- Experimental Setup : Ligand Exposure | Duration: 7 Days
- Significance : Authors have indicated that results with Score.1 (STARS Score) > 0.0 AND Score.2 (FDR) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- This CRISPR screen identified genes that showed resistance to interferon gamma (STARS analysis at FDR < 10%) and none that showed increased sensitivity.
- The authors note the following: p-value=0 means <5.97728631202e-06, FDR=0 means <0.00131158739647
Score Distribution
< 10.57302728
> 0.5473481492