CRISPR Screen Dataset
Koizumi Y (2019) - 1-PMID30734978
Title: Genome-Scale CRISPR/Cas9 Screening Reveals Squalene Epoxidase as a Susceptibility Factor for Cytotoxicity of Malformin A1.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Lymphoma Cell Line [BTO:0000104]
- Cell Line : U-937 [BTO:0001412, CELLOSAURUS:CVCL_0007]
- Phenotype : Response To Chemicals
- Condition : Malformin A1 (1.0 µM) [ChemSpider]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Kolmogorov-Smirnov | Number of Hits: 100 | Full Dataset Size: 19,052
- Experimental Setup : Drug Exposure | Duration: 7 Days | MOI: = 0.5
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- Authors screened for genes whose KO conferred increased proliferation in the presence of Malformin 1A
Score Distribution
< 0.00647832
> 0.0000370361