CRISPR Screen Dataset
Zimmermann M (2018) - 3-PMID29973717
Title: CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions.
Screen Details:
- Screen Rationale : Increased sensitivity to drug
- Cell Type : Retinal Pigment Epithelium Cell Line [BTO:0002334]
- Cell Line : hTERT-RPE1 [BTO:0004790, CELLOSAURUS:CVCL_4388]
- Phenotype : Response To Chemicals
- Condition : Olaparib (0.5 µM) [PubChem]
- Library : TKO (Toronto Knockout) v1 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 61 | Full Dataset Size: 15,463
- Experimental Setup : Drug Exposure | Duration: 15 Days | MOI: ~ 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for genes whose KO resulted in decreased cell proliferation in the presence of olaparib
- High confidence hits (FDR<=1% + >=2 cell lines at FDR<=10%)
- Mutants are enhancers of PARP-inhibition cytotoxicity
Score Distribution
< 7.13
> -7.186