CRISPR Screen Dataset
Han K (2020) - 11-PMID32238925
Title: CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities.
Screen Details:
- Screen Rationale : Cell-essential genes and growth restrictive genes in spheroid growth conditions compared to mono-layer growth conditions
- Cell Type : Non-Small Cell Lung Cancer Cell Line [BTO:0002553]
- Cell Line : NCI-H1975 [BTO:0004824, CELLOSAURUS:CVCL_1511]
- Phenotype : Cell Proliferation
- Condition : 3D spheroid growth
- Library : Bassik Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Phenotype scores based on log2 fold enrichments | Number of Hits: 332 | Full Dataset Size: 19,828
- Experimental Setup : Timecourse | Duration: 21 Days | MOI: ~ 0.4
- Significance : Authors have indicated that results with Score.1 (T-score) < -2.5 OR Score.1 (T-score) > 3.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors identified genes with differential effects in 3D growth conditions by normalizing 3D phenotypes against the corresponding 2D phenotypes (3D/2D).
- KO of genes with a positive T-score results in increased proliferation, suggesting the gene normally acts in a growth restrictive manner. KO of genes with a negative score results in decreased proliferation, suggesting the gene normally acts in a cell-essential manner
Score Distribution
< 13.5
> -5.95