CRISPR Screen Dataset
Lai Y (2020) - 2-PMID32970993
Title: Illuminating Host-Mycobacterial Interactions with Genome-wide CRISPR Knockout and CRISPRi Screens.
Screen Details:
- Screen Rationale : Gene inhibition affects response to bacteria
- Cell Type : Monocytic Leukemia Cell Line [BTO:0002332]
- Cell Line : THP-1 [BTO:0001370, CELLOSAURUS:CVCL_0006]
- Phenotype : Response To Bacteria
- Condition : Mycobacterium tuberculosis variant bovis BCG (10 MOI) [NCBI Taxid 33892]
- Library : Human CRISPRi sgRNA library Dolcetto [AddGene, AddGene, AddGene] | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 491 | Full Dataset Size: 18,901
- Experimental Setup : Bacteria Exposure | Duration: 3 Days | MOI: = 0.3
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- Inhibition of the hit gene results in increased resistance to Mycobacterium tuberculosis variant bovis BCG
Score Distribution
< 13.2936005
> 1.001803853