CRISPR Screen Dataset
Pacheco AR (2018) - 1-PMID29921669
Title: CRISPR Screen Reveals that EHEC's T3SS and Shiga Toxin Rely on Shared Host Factors for Infection.
Screen Details:
- Screen Rationale : Resistance to bacteria
- Cell Type : Colonic Adenocarcinoma Cell Line [BTO:0001913]
- Cell Line : HT-29 [BTO:0000182, CELLOSAURUS:CVCL_0320]
- Phenotype : Cell Proliferation
- Condition : Escherichia coli (str. EDL933) (100.0 MOI) [NCBI Taxid 155864]
- Library : Avana | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : STARS | Number of Hits: 20 | Full Dataset Size: 18,675
- Experimental Setup : Bacteria Exposure | Duration: 5 Days
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.001 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes causes increased resistance to Enterohemorrhagic Escherichia coli with an espZ mutation which heightens T3SS activity and increases host cell death associated with infection
Score Distribution
< 16.6971
> 0.829151