CRISPR Screen Dataset
DeJesus R (2016) - 2-PMID27351204
Title: Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62.
Screen Details:
- Screen Rationale : Positive regulators of SQSTM1
- Cell Type : Glioma Cell Line [BTO:0000711]
- Cell Line : H4 [BTO:0003704, CELLOSAURUS:CVCL_1239]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : CRISPRn (Hoffman and Nyfeler, 2016) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 102 | Full Dataset Size: 18,007
- Experimental Setup : Timecourse | Duration: 7 Days | MOI: = 0.5
- Significance : Authors have indicated that results with Score.1 (Log2FC) >= 1.35 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit genes resulted in decreased SQSTM1 expression as measured by FACs sorting of a GFP tagged SQSTM1
Score Distribution
< 3.01
> -1.55