CRISPR Screen Dataset
Park RJ (2017) - 1-PMID27992415
Title: A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors.
Screen Details:
- Screen Rationale : Response to virus (host dependency factors)
- Cell Type : Lymphoma Or Leukaemia Cell Line [EFO:0002937]
- Cell Line : GXRCas9 [CELLOSAURUS:CVCL_C8ZC]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Virus: HIV (Human immunodeficiency virus) (0.025 MOI) [NCBI Taxid 12721]
- Library : Human Activity-optimized genome-wide library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 5 | Full Dataset Size: 18,387
- Experimental Setup : Virus Exposure | Duration: 6 Weeks
- Significance : Authors have indicated that results with Score.1 (Log2FC) > 2.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors screened CD4+ T cells in which HIV infection leads to GFP expression and used FACs sorting to identify host genes important in facilitating HIV infection
Score Distribution
< 11.748
> -4.568