CRISPR Screen Dataset
Richardson RB (2018) - 1-PMID30224801
Title: A CRISPR screen identifies IFI6 as an ER-resident interferon effector that blocks flavivirus replication.
Screen Details:
- Screen Rationale : Genes involved in interferon immune reponse
- Cell Type : Huh-7 Cell [BTO:0001950]
- Cell Line : Huh-7.5 [BTO:0004126, CELLOSAURUS:CVCL_7927]
- Phenotype : Response To Virus
- Condition : Virus: Yellow Fever Virus 17D (1 MOI) [NCBI Taxid 11090]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 17 | Full Dataset Size: 19,112
- Experimental Setup : Virus Exposure | Duration: 2 Days
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors use yellow fever virus expressing Venus (YFV-Venus) to sort cells that are infected despite treatment with a highly suppressive dose of IFN-a
Score Distribution
< 0.99999
> 0.000000000000251